Expression of CLL signature genes by ROR1^Negor ROR1^PosCLL cells. Representative histograms depicting the fluorescence of CD19⁺ cells labeled with Alexa-647–conjugated 4A5 (anti-ROR1 mAb) (shaded histograms) or Alexa-647-conjugated nonspecific immunoglobulin G (IgG) of the same isotype (open histograms) for (A) ROR1^Pos CLL B cells, (B) ROR1^Neg CLL cells, or (C) representative normal blood B cells. The case identifier and the ROR1 ΔMFI are indicated at the top right corner of each panel. (D) Immunoblot analyses of whole cell lysates of ROR1^Neg CLL or ROR1^Pos CLL, as indicated at the top. Each lane represents a separate case. The membranes were probed with mAbs specific for ROR1 (top row) or β-actin (bottom row), as indicated on the left. (E-F) Each column represents a separate case of ROR1^Neg or ROR1^Pos CLL, as indicated at the top. The relative expression of the genes indicated on the right margin are provided in rows, using the color coding for log₂ normalized effective count z scores as per the scale provided at the bottom of each heat map. (E) Heat map for 34 genes previously found expressed differentially by CLL cells vs normal B cells. (F) Heat map for 65 genes previously found expressed differentially by CLL cells vs diffuse large cell lymphoma (DLCL) cells or normal germinal center (GC) B cells. (G) Relative amount of TCL1A transcripts found in ROR1^Neg or ROR1^Pos CLL cases, as indicated at the bottom of the graph. Each dot represents the relative TCL1A of a separate case. The large horizontal bar indicates the mean level of TCL1A transcripts, and the 2 smaller horizontal bars show the 95% confidence interval.