Functional mitochondria enter into AML cells. Confocal microscopy images of MS-5-Omi-mCherry cells cocultured with U937-GFP cells. Recorded fluorescence is indicated in the top left angle. (A) Representative cells are shown in Z-stack overlay mode. Mitochondria were evidenced using either anti-cytochrome C or anti-Tom20 antibodies. (B) Specific stack analysis. AML cell limits (yellow line) were defined by subtracting the background with ImageJ software. Single and double staining are displayed. (C) Orthogonal views and (D) 3D reconstruction images of Omi/cytochrome C and Omi/Tom20 stainings. All images: 60× enlargement; scale bar, 1 µm.