Characterization of cultivated BM EPCs. (A) Representative images for cultivated BM EPCs at day 7 in culture among subjects with PGF and GGF and normal controls (Normal). Typical BM EPCs were characterized by double positive staining (merged in yellow) with both DiI-AcLDL (red) and FITC-UEA-I (green) (original magnification ×20). (B) Quantification of double positive staining cells (merged in yellow) with both DiI-AcLDL (red) and FITC-UEA-I (green) at day 7 in culture among the PGF, GGF, and normal control groups (original magnification ×10). Data are expressed as the mean ± SEM. (C) The typical EPCs phenotype of cultivated BM EPCs was confirmed by demonstrating the similar expression of CD34, CD309, and CD133 at day 7 in culture by flow cytometry among subjects with PGF, subjects with GGF, and normal controls. Aliquots of isotype-identical antibodies served as negative controls.