Comparison of BeneFIX and Alprolix at different doses in Hemophilia B mice. Each point in (A) represents the number of times that clotting can recur in a hemophilic mouse with a saphenous vein injury 7 days postinfusion with either Alprolix or BeneFIX. The P values shown within the plot are from Mann-Whitney tests. (B) Plot of median values for the number of clots formed at different doses of BeneFIX or Alprolix. The medians were taken from the data shown in (A) together with additional similar data from higher doses. The line connecting the points has no theoretical meaning but serves to draw attention to the proportional increase. (C) Mice expressing FIX_K5A at 120% of WT levels are about 50% as active in the saphenous vein bleeding model as are WT mice. The in vitro activity of FIX_K5A is indistinguishable from FIX_WT in an activated partial thromboplastin time assay. HemB, hemophilia B.