Overview of the mouse xenograft leukemia models. (A) Schematic representation of the generation of muBM-iv and huBM-sc models. (B) Representative hematoxylin and eosin (H&E) stain of scaffold sections 6 weeks after implantation. b, bone; s, scaffold; v, blood vessels. (C) Success rate of leukemia development in muBM-iv and huBM-sc models, expressed as percentage of AML samples from different risk groups, which engrafted or not in the 2 xenograft models. (D) Scatter plots showing engraftment of donor human CD45+ cells in different compartments of the mouse at euthanasia. Engraftment values from several mice for each AML sample are expressed as mean ± standard error of the mean (SEM). Route 1: cells were directly injected into the humanized scaffolds; route 2: cells were injected intravenously into mice without humanized scaffolds. (E) Experiment as in (D), but now cells were injected intravenously into mice carrying humanized scaffolds. (F) In vivo tumor growth rates of the biggest tumor scaffold in each mouse. Each colored line represents 1 mouse from a certain AML sample. Tumor size (cm3) was used as the end point of the experimental period. int, intermediate; muSp, murine spleen; muLv, murine liver; muPB, murine peripheral blood; n.e., no engraftment.
