Hyperfibrinolytic state induces BBB leakage of EB. (A) Representative euglobulin lysis time assay from plasma samples of pLIVE-0- and pLIVE-tPA-transfected mice (harvested 48 hours after hydrodynamic transfection). (B) Corresponding quantification of the half-lysis time (n = 5 per group). (C) Experimental design performed to study BBB permeability using NIRF techniques. EB extravasation was measured ex vivo 48 hours after pLIVE (0 or tPA) transfection in the brain and the spinal cord. (D) Representative pictures of ex vivo brains after pLIVE (0 or tPA) transfection. (E) Brain EB permeability was quantified by NIRF. (F) Normalized mean fluorescence quantification of D (n = 5 per group). (G) Ex vivo representative images of the brain and spinal cords after pLIVE transfection. (H) Normalized mean fluorescence quantification of spinal cord EB extravasation (n = 5 per group). (I) Representative epifluorescence images of pLIVE-tPA-transfected animals, showing EB extravasation (red) in the cortex and the cerebellum, but not in the spinal cord. *P < .05 vs control; ns = nonsignificant differences.