O-fucosylation and C-mannosylation sites on ADAMTS13. (A) Schematic representation of the ADAMTS13 molecule with the O-fucosylation sites depicted as red triangles and blue circles (fucose and glucose residues); green circles represent the mannose residues. In total, 3 C-mannosylation sites were identified by the Byonic software. Eight O-fucosylation sites, all of them modified by a glucose-fucose group attached to either a serine or a threonine, were identified. (B) Amino acid position and corresponding domain of ADAMTS13 and attached glycan structures (Hex either being a mannose or a glucose, and dHex being a fucose moiety) are depicted.