Identification of an adenosine receptor-independent component of the ticagrelor-induced increase in platelet VASP-P and cAMP. Representative immunoblots and bar/x-y charts showing VASP-P, VASP, and α-tubulin (loading control) levels in washed platelets after incubation at 37°C with the following: (A-B) Vehicle (0.1% DMSO for 60 minutes) or 10 µM ticagrelor for 0.5, 5, 10, 15, 30, and 60 minutes following preincubation of all samples (except vehicle control) with XAC (10 µM, at 37°C) for 90 minutes; n = 3. (C-D) Vehicle (0.1% DMSO), 0.1, 0.3, 1, 3, and 10 µM ticagrelor for 60 minutes following preincubation of all samples (except vehicle control) with XAC (10 µM at 37°C) for 90 minutes; n = 5. (E-F) No drug, vehicle (0.1% DMSO), ticagrelor (10 µM), and NECA (1 µM) for 60 minutes following preincubation of all samples (except vehicle control) with XAC (10 µM at 37°C) for 90 minutes; n = 5. (G-H) Vehicle (0.1% DMSO), ticagrelor (10 µM), AR-C 66096 (10 µM), PSB 0739 (10 µM), and R-138727 (10 µM) for 60 minutes. *P < .05; **P < .01; ***P < .001; ns, P > .05. Kilodaltons of molecular size markers are shown on left of blots.