Time- and concentration-dependence of increases in platelet VASP-P. Representative immunoblots and bar/x-y charts showing VASP-P, VASP, and α-tubulin (internal control) levels in washed platelets after preincubation at 37°C with the following: (A-B) Ticagrelor (10 µM) for 0, 0.5, 5, 10, 15, 30, and 60 minutes; n = 5. (C-D) Vehicle (0.1% DMSO) and 0.1, 0.3, 1, 3, and 10 µM ticagrelor for 60 minutes; n = 5. (E-F) Untreated, vehicle (0.1% DMSO), ticagrelor (10 µM), AR-C 66096 (10 µM), PSB 0739 (10 µM), and R-138727 (10 µM) for 60 minutes; n = 4. (G-H) Untreated, vehicle (0.1% DMSO), ticagrelor (10 µM), and NECA (µM) for 60 minutes following preincubation at 37°C for 30 minutes with untreated, vehicle (0.1% DMSO), or XAC (1 µM); n = 6. Data displayed as mean and SEM. **P < .01; ***P < .001; ns, P > .05. Statistical analysis used was 2-way ANOVA (H). Kilodaltons of molecular size markers are reported on the left.