Microarray analysis shows partial maturation of IFN-α–treated AGM HSCs. (A) MDS shows 3 distinct groups of embryonic HSCs maturation, which correspond to AGM, “transition,” and FL signatures. IFN-α–treated AGM HSCs and subset of E12.5 FL HSCs are grouped as having this “transition” signature. Data from this study is depicted by open circles, whereas data from McKinney-Freeman et al (GSE37000) is depicted by closed circles. (B) Intersection of differentially expressed genes between E13.5-14.5 FL HSCs and AGM, and between AGM and IFN-α–treated AGM (nominal P value cutoff .05). (C) Top 5 KEGG pathway terms identified from FL upregulated and IFN-α–regulated gene set from (B). (D) Top 5 KEGG pathway terms identified from FL downregulated and IFN-α–regulated gene set from (B). MDS, multidimensional scaling.