Minihepcidin M009 did not impair the ability of DFP to reduce iron overload in a mouse model of non–transfusion-dependent thalassemia. DFP alone (added into the drinking water at concentration of 1.25 mg/mL) caused a significant reduction in liver iron content, whereas the combination of DFP with minihepcidin M009 (used at a dose of 2.625 mg/kg) did not impair the ability of DFP to reduce organ iron content when compared with vehicle-treated animals (A). Animals treated with DFP showed a trend toward increased TSAT that was instead decreased in animals treated with M009 (B). ELISA assay showed reduction of serum Erfe levels (C). Results represent mean ± SD; **P < .01, *P < .05. Analysis was performed using one-way ANOVA with Tukey multiple comparison adjustment. WT data are displayed as a reference guide but are not included in the between-group test comparisons.