WDR1 knockdown increases PLP size and adhesion but decreases PLP production. (A) Expression of CD42b, CD41, and CD61 platelet markers at the PLP surface analyzed by flow cytometry in WDR1 KD and control PLPs. (B) Forward side scatter (FSC) of PLPs produced by WDR1 KD and control MEG-01 cells. WDR1 KD-derived PLPs are larger than PLPs derived from controls. Similar results were obtained in an additional 2 experiments. (C) Quantification of PLP production by WDR1 KD and control MEG-01 cells (P = .02, Student t test). (D) Quantification of PLP adhesion upon stimulation with collagen and thrombin. WDR1 KD-derived PLPs are more than threefold more adherent than their control (P = .003, Student t test). Data are mean ± SEM of 3 experiments. *P < .05 vs control MEG-01-derived PLPs. SSC-A, side scatter.