WDR1 regulates actin cytoskeleton dynamics. (A) F-actin staining with phalloidin of adherent WDR1 KD or control cells 12 hours after stimulation with or without thrombin 1 U/mL on collagen-coated coverslips. In the bottom right panel, WDR1 KD MEG-01 cells were also treated with cytochalasin B, an inhibitor of actin polymerization. F-actin (green), 4′,6-diamidino-2-phenylindole (blue). (B) Unactivated WDR1 KD or control MEG-01 cells stained with phalloidin-fluorescein isothiocyanate were analyzed on flow cytometry. Data are mean ± SEM of 3 experiments (P = .02, Student t test). (C) Comparison of F-actin-to-G-actin ratio in control and WDR1 KD MEG-01 (left panel) based on a immunoblot analysis (right panel). Data are mean ± SEM of 3 experiments (P = .04, Student t test). *P < .05 vs control MEG-01 cells. MFI, mean fluorescence intensity.