American Society of Hematology

Figure 1

$Figure 1. Components of microbiota and immunological assay. (A) Temporal dynamics of the microbiota (at the genus level) and clinical course in each patient: (i) case 1, (ii) case 2, (iii) case 3, and (iv) case 4. *1: Data from the day after first FMT could not be obtained because of the lack of fecal sample. (B) (i) Subpopulation of Tregs. Tregs can be dissected into 3 subpopulations by expression levels of FoxP3, CD45RA. FoxP3loCD45RA+ cells (fraction 1), designated as naive Tregs, which differentiate into eTregs under antigenic stimulation; FoxP3hiCD45RA− cells (fraction 2), designated eTregs, which are terminally differentiated and highly suppressive; and FoxP3loCD45RA− non-Tregs (fraction 3), which do not possess suppressive activity, but secrete proinflammatory cytokines.20 (ii) The absolute number of eTregs (red lines) and the eTreg/CD8+ T-cell ratio (green lines) in peripheral blood of each patient. CAZ, ceftazidime; CFPM, cefepime; FK, tacrolimus; Fr, fraction; LVFX, levofloxacin; MEPM, meropenem; PSL, prednisolone; ST, sulfamethoxazole/trimethoprim; TAZ/PIPC, tazobactam/piperacillin; TEIC, teicoplanin; VCM, vancomycin.$

Components of microbiota and immunological assay. (A) Temporal dynamics of the microbiota (at the genus level) and clinical course in each patient: (i) case 1, (ii) case 2, (iii) case 3, and (iv) case 4. *1: Data from the day after first FMT could not be obtained because of the lack of fecal sample. (B) (i) Subpopulation of Tregs. Tregs can be dissected into 3 subpopulations by expression levels of FoxP3, CD45RA. FoxP3^loCD45RA⁺ cells (fraction 1), designated as naive Tregs, which differentiate into eTregs under antigenic stimulation; FoxP3^hiCD45RA⁻ cells (fraction 2), designated eTregs, which are terminally differentiated and highly suppressive; and FoxP3^loCD45RA⁻ non-Tregs (fraction 3), which do not possess suppressive activity, but secrete proinflammatory cytokines.²⁰ (ii) The absolute number of eTregs (red lines) and the eTreg/CD8⁺ T-cell ratio (green lines) in peripheral blood of each patient. CAZ, ceftazidime; CFPM, cefepime; FK, tacrolimus; Fr, fraction; LVFX, levofloxacin; MEPM, meropenem; PSL, prednisolone; ST, sulfamethoxazole/trimethoprim; TAZ/PIPC, tazobactam/piperacillin; TEIC, teicoplanin; VCM, vancomycin.

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