Surface expression of adhesion and signaling molecules on aged neutrophils. Using multichannel flow cytometry, surface expression of different adhesion and signaling molecules on aged neutrophils was analyzed as detailed in “Methods.” Quantitative data for aged and nonaged neutrophils isolated from the peripheral blood of WT mice undergoing exposure to PBS or LPS (A, B), as well as to the CXCR4 inhibitor AMD3100, the TLR-4 inhibitor TAK-242, or the MAPK inhibitor compounds FR180204 (ERK 1/2 MAPK), SB203580 (p38 MAPK), or SP600125 (JNK MAPK) (C). Mean ± SEM for n = 4-6; ^#P < .05 vs nonaged neutrophils; *P < .05 vs PBS; ^§P < .05 vs drug vehicle. RFI, relative fluorescence intensity.