Kinetics of anti-HLA class II allele-specific immune reactivity in early post-dUCBT PB samples. Patient PBMCs were propagated and assayed as described in Figure 2. (A) Immune reactivity toward individual alleles (n = 33) tested in 19 PBMC samples as a function of PBMC sampling time after dUCBT; individual and median (line) values are shown. (B) Immune reactivity toward individual alleles in paired PBMC samples of 5 patients: sample 1, 1 month (median; range, 1-2 months) after dUCBT; sample 2, 2 months (median; range, 2-4 months) after dUCBT. Individual and median (line) values are shown; P value, paired Student t test.