Characteristics of post-dUCBT patient PBMC samples before and after HLA class II allele-specific T-cell propagation. (A) Absolute numbers of CD3⁺ T cells in PB samples analyzed for HLA class II allele immune recognition. Graphs represent individual and median (line) values. Dotted line, lower limit of normal values of CD3⁺ T cells in blood of healthy individuals. (B) Percentage of CD4⁺ T cells in PB (before) and in T-cell cultures after HLA class II–specific amplification, with either HeLa cells transduced with HLA class II mismatched alleles (after, MM) or with HLA class II third-party alleles (after, 3P). Depending on the number of mismatched alleles in the dUCBT combination, from 1 to 7 T-cell amplification cultures per PB sample were performed. Graphs represent individual and median (line) observations. Gray area, normal range of CD3⁺CD4⁺ values in blood of healthy individuals; significance in nonpaired t tests is shown. (C) Increase in T-cell numbers in post-dUCBT PBMC samples following processing in HLA class II–specific amplification cultures, expressed as fold increase relative to the T-cell number at start of culture (see B).