Suppression of DNMAML-GFP improves survival, relieves cell cycle arrest, and increases LIC frequency. Sorted DNMAML-expressing thymic lymphoma cells from LCDDR mice were transferred into secondary recipients. T-ALLs that developed in these mice were subdivided into T-ALL cells that continued to express DNMAML-GFP (DNMAML⁺) and T-ALL cells that suppressed DNMAML-GFP (DNMAML⁻). These 2 populations were then analyzed for T-cell markers (CD4 and CD8), apoptotic cells (Annexin V⁺/7-AAD⁻), and cell cycle status using flow cytometry. A representative recipient mouse is shown in panel A and the rest of the cohort is shown in panel B in scatter plot format. P values (Student t test) were .0002 (% Annexin V⁺/7-AAD⁻), .0004 (% G0/G1), <.0001 (% S), and <.0001 (% G2M) and are shown on the plots. (C) Sorted DNMAML-GFP⁺ or DNMAML-GFP⁻ thymic lymphoma cells were transferred at limiting dilution into lethally irradiated secondary recipient mice. The number of mice in each cohort that developed T-ALL over at least 6 months was used to determine the frequency of LIC activity by Poisson statistics. C.I., confidence interval.