The pan-Notch inhibitor DNMAML-GFP is expressed in most T cells beginning at the DN3 stage. Flow cytometric analysis of DN T-cell development and DNMAML-GFP expression in DN2 (CD44⁺CD25⁺Lin⁻), DN3 (CD44⁻CD25⁺Lin⁻), and DN4 (CD44⁻CD25⁻Lin⁻) subsets at 5 to 7 weeks of age in the 6 different mouse strains used in the study (Table 1). Flow cytometric analysis of thymic development beyond the DN T-cell stage and expression of the DNMAML-GFP genetic inhibitor in ISP (CD8⁺TCRβ⁻), DP (CD4⁺CD8⁺), mature CD8 (CD8⁺TCRβ⁺), and mature CD4 (CD4⁺TCRβ⁺) subsets in the 6 different mouse strains used in the study. See supplemental Figure 1 for absolute numbers.