Deletion of Moz results in a substantial reduction in populations with a cell surface phenotype characteristic of HSCs and of cells in G₀. (A) Experimental design. Mice were treated with poly(I:C) and then left undisturbed for 4 months to allow recovery from acute inflammation induced by poly(I:C). (B) Examples of PCR genotyping of progenitor colonies from Moz^fl/fl and Moz^fl/fl;Mx1-cre cultures after stimulation with SCF, EPO, and IL3. Note that myeloid colony-forming progenitors (supplemental Figure 3) are derived from Moz-deleted cells that have existed for 4 months. (C) Flow cytometry plots showing analyses of the LSK-CD34^–FLT3^– and LSK-CD48^–CD150⁺ populations in Moz^fl/fl and Moz^fl/fl;Mx1-cre bone marrow cells. (D-E) Quantification of stem cell surface markers by flow cytometry of recipients of Moz^fl/fl and Moz^fl/fl;Mx1-cre cells. Note the absence of typical LSK-CD34^–FLT3^– and LSK-CD48^–CD150⁺ populations. (F) Cell cycle analysis of stem/progenitor populations. Note the reduction in the number of cells in G₀. Data are presented as the mean ± SEM.