Morphology of neutrophils. (A) Neutrophils from whole blood smears of a normal subject (NL, top row) and patients I.2.2 (middle row) and I.2.3 (bottom row) stained with Diff-Quik. The black arrows indicate regions of abnormally agranular cytoplasm. (B) Neutrophils prepared by Ficoll centrifugation, 3% dextran sedimentation, and sequential erythrocyte hypotonic lysis. Neutrophils were centrifuged onto slides at 100g for 10 minutes and then stained. Red arrows indicate herniated nuclear lobes. (C) Transmission electron micrographs of isolated neutrophils. Black arrows indicate areas of abnormally agranular cytoplasm; red arrows indicate herniated nuclear lobes. Overall, 40% to 60% of patient cells had abnormal morphology. (D) Scanning electron micrographs of isolated neutrophils, showing knob-like projections on patient neutrophils (magnification, ×6000). (E) Scanning electron micrographs of isolated neutrophils. Stimulation of normal cells with fMLF resulted in polarization with elongation of the cells with protruding lamellipodia and trailing uropod. Although neutrophils from patients I.2.2 and I.2.3 underwent some morphology change, they failed to exhibit the morphological changes of normal subjects.