Brg1 regulates the proliferation in hematopoietic progenitors. (A) The BM of mice reconstituted with HSPCs transduced of empty-RV or shBrg1-RV was analyzed using GFP expression by flow cytometry. (B) Brg1 expression in LSK cells was measured using quantitative RT-PCR analysis (N = 3). (C) Representative profiles of lineage-committed progenitors with surface expression of CD34 and FcγRIII in LK population from Brg1-knockdown mice. Average frequency of GMP (CD34⁺FcγRIII^hi) in LK subsets is depicted (right). (D) Representative flow cytometric profiles of LSK (Lin⁻c-Kit⁺Sca-1⁺) and LK (Lin⁻c-Kit⁺Sca-1⁻) subpopulations (left) and average frequency of LSK subsets in Brg1-knockdown mice (right). (E) BrdU-positive cells were analyzed in LSK and GMP subsets. Data are representative of 2 independent experiments. (F) Expression of p21, p27, and Brg1 was measured in K562 cells treated with short interfering (siRNA) targeting Brg1. Data represent mean ± SEM (N = 3). (G) Expression of cell cycle–related genes was analyzed in K562 cells treated with siRNA targeting Brg1. Results are presented relative to those of cells transfected with control siRNA targeting GFP (N = 3). (H) Gene expression was analyzed in LSKs purified from Brg1-knockdown mice. Results are presented relative to those of cells transfected with control siRNA targeting GP (N = 3). (I) Representative flow cytometric analysis (upper panels) and average frequency of granulocytes (lower panels) in the BM from reconstituted mice as described in the figure. Upper panels, empty-NGFR and empty-GFP; middle panels, miR-139T-NGFR and empty-GFP; lower panels, miR-139T-NGFR and shBrg1-GFP. (J) Cell proliferation was analyzed in the subsets of LSK and GMP of reconstituted mice as described in the figure using 5-bromo-2′-deoxyuridine (BrdU) pulse labeling. Data are representative of 2 independent experiments.