Knockdown of miR-139-5p in HSC/progenitor causes an increase in myeloid lineage cells. (A) The BM of mice reconstituted with HSPCs transduced by empty-RV or miR-139T-RV was analyzed using GFP expression by flow cytometry. The percentage of GFP-positive cells is indicated. Gray, GFP expression in nontransduced control BM from C57BL/6 mice; black line, GFP expression in transduced BM. (B) The expression of miR-139-5p was measured in LSK and Lin⁻c-Kit⁺Sca-1⁻ (LK) cells purified from reconstituted mice. Data represent the mean ± SEM (N = 3). (C) Representative flow cytometric analysis of granulocytes (Gr-1⁺Mac-1⁺) and B cells (B220⁺Mac-1⁻) in the BM gated on GFP-positive populations, with surface markers Gr-1, Mac-1, and B220 (upper panels). Results plotted as average frequency of granulocytes or B cells (right panels). Each individual dot represents 1 mouse. (D) Representative flow cytometric analysis of splenocytes from reconstituted mice, with Gr-1 and Mac-1 (left) and average frequency of Gr-1⁺Mac-1⁺ granulocytes (right). Each individual dot represents 1 mouse.