TNFα/TNFR2 disruption using anti-TNFR2 blocking mAb increases inflammatory cytokine production by donor CD4 and CD8 T cells. [B6 x C3H]F1 female mice underwent TBI followed by transplantation with B6 BM cells plus T cells treated or not with anti-TNFR2 administered at day 0, 2, and 4. Mice were euthanized, and donor CD4⁺ and CD8⁺ T cells were analyzed at day 14 posttransplantation in the spleen of grafted animals. Mean absolute numbers of splenocytes and percentage of CD4⁺ and CD8⁺ donor T cells are determined as well as intracellular IFNγ and TNFα production. Each plot represents a mouse; Student t test analysis was performed to compare the anti-TNFR2 mAb effect on T cells. *P < .05; **P < .01; ***P < .001.