Degradation of platelet RNA. (A) Correlation of the change in gene expression between megakaryocte polyA⁺ RNA and platelet polyA⁺ RNA (y-axis), and mRNA half-life estimates (x-axis). Distributions of both are shown as histograms on each axis. (B) qPCR analysis of differential decay of linear and circRNAs in platelets following incubation at 37°C for 0, 72, and 96 hours. Data from 3 biological replicates are shown. (Top left) Expression levels of housekeeping genes (CT values). (Top right) Expression levels of linear structures from 5 circRNA-producing genes relative to the housekeeping pool (−∆CT values). (Bottom) Expression levels of 7 circRNAs relative to linear transcripts from the same loci, both normalized to the housekeeping pool (−∆∆CT values).