Confirmation of circRNA abundance and resistance to RNAse R. (A) Schema of qPCR assays using an E5-E2 circRNA as an example. All assays use a common reporter probe and use either an exon downstream of the circRNA to assay linear expression (probe in donor exon) or an exon upstream (probe in acceptor exon). (B) (Left) Expression levels (−∆CT values) of linear (Ex1-2) and circular (Ex5-2 and Ex4-2) MAN1A2 transcripts relative to housekeeping pool. (Right) Expression levels of circRNAs relative to linear RNAs from the same loci normalized to housekeeping pool (−∆∆CT values). (C) Expression of 9 circRNAs relative to linear forms from the same loci, normalized to housekeeping pool (−∆∆CT values). (D) Change in CT values of circRNAs relative to linear forms from the same loci in RNAse R digested RNAs, normalized to mock digested RNAs (−∆∆CT values). Templates, circRNAs, and linear forms assayed are indicated.