American Society of Hematology

Figure 1

$Figure 1. Identification of the type I CALR mutation in the granulocyte fraction of both twins. (A) Results from next-generation sequencing showing the presence of the same 52-bp deletion in the coding region of the CALR gene in DNA obtained from granulocytes but not from CD3+ lymphocytes. (B) DNA fragment analysis using a 5′-fluorescein–labeled primer confirmed the presence of 27% and 9.7% mutant alleles in the granulocyte component in both sisters, while absent in CD3+ lymphocytes.$

Identification of the type I CALR mutation in the granulocyte fraction of both twins. (A) Results from next-generation sequencing showing the presence of the same 52-bp deletion in the coding region of the CALR gene in DNA obtained from granulocytes but not from CD3⁺ lymphocytes. (B) DNA fragment analysis using a 5′-fluorescein–labeled primer confirmed the presence of 27% and 9.7% mutant alleles in the granulocyte component in both sisters, while absent in CD3⁺ lymphocytes.

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