Figure 4
![Figure 4. FXIII-A2B2 binds fibrinogen residues γ390-396 via the FXIII-B subunits. rFXIII-A2 (10 µg/mL [60 nM], final), rFXIII-B2 (10 µg/mL [62 nM], final), or both, were mixed with (A) γA/γA or (B) Fibγ390-396A fibrinogen (1 mg/mL [2.9 μM], final) and precipitated with glycine. (A-B) Representative western blots for fibrinogen (Fgn), FXIII-A, and FXIII-B in the initial sample (I), pellet (P), or supernatant (S). Note that P and S samples were prepared after the addition of glycine and are therefore diluted relative to the I sample. Blots are representative of N = 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/15/10.1182_blood-2016-04-712323/4/1969f4.jpeg?Expires=1733866646&Signature=tP6xD6di~jsDAXsOTQySp3PAju6CvaxrY9llkPRCkRKK8vsNwtyXmwUUcy82VWmqskAiufHfQFz~p6mR0sc9xXemOxnnU0pKQrIzX6whqPFyOD7OWB0IcZKE9VI0krhDo-yRyGGpiRYpJIJRWPczQCV4GghuMyU6tJy6kQw2NS5EbN8eLikN4jN8LYZl3FCXEocyRFSmb-Y8BwOwRieru0iUsgjRNkZC87kXatOfbJKf5NKQ3RmYp1LMdgh0sfnI5UMbYHrtEVOhiFXeRpT7Dl7pk55i2~R~92otBMlKiBidpZfuSphTvkDVEiQBEoiyJsj5V8SBnU-2pTsKyQ3TKw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FXIII-A2B2 binds fibrinogen residues γ390-396 via the FXIII-B subunits. rFXIII-A2 (10 µg/mL [60 nM], final), rFXIII-B2 (10 µg/mL [62 nM], final), or both, were mixed with (A) γA/γA or (B) Fibγ390-396A fibrinogen (1 mg/mL [2.9 μM], final) and precipitated with glycine. (A-B) Representative western blots for fibrinogen (Fgn), FXIII-A, and FXIII-B in the initial sample (I), pellet (P), or supernatant (S). Note that P and S samples were prepared after the addition of glycine and are therefore diluted relative to the I sample. Blots are representative of N = 3 experiments.
