Figure 1
![Figure 1. Fibrinogen residues γ390-396 are necessary for FXIII-A2B2 binding. Recombinant human fibrinogen variants (γA/γA, γ′/γ′, Aα251, and Fibγ390-396A) were mixed with FXIII-A2B2 (1 mg/mL [2.9 μM] and 20 µg/mL [60 nM], final, respectively) and precipitated with glycine. (A) Representative western blots for fibrinogen (Fgn), FXIII-A, and FXIII-B in the initial sample (I), pellet (P), or supernatant (S). Note that P and S samples were prepared after the addition of glycine and are therefore diluted relative to the I sample. (B) Quantitation of all blots, indicating percent of FXIII-A or FXIII-B in the pellet, relative to total FXIII in the pellet and supernatant. Bars are means ± SEM; N = 3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/15/10.1182_blood-2016-04-712323/4/1969f1.jpeg?Expires=1733866823&Signature=lLJAIn~AMOsNW-oXvHlgRV8EG5DPoZYe-bEvmJWxbaQug0WmQeTl~RzPj0EcnQS29IZH22RbOCuyc~WG6GCgJSJxnEz3bM9xw2Xi1kuE4kww6glHDENMGSaj4KVNb7YzxprkKWJeYAJqtiW5zHDHuc9VDlBG0ZR-hLqYkxoiDzYI3koq9D0BSEC4ldzp8WMzxsFbcUa4aVOxq1kIfy5ZZ4o6dJL154rD1IxI4Vou6UG7UknDqilOtnNSyBoB-zVbSRkR3ml66h96XfV-9vWjj3ua11XBBldP6r8J-KaTCfbOqx0i412PneYBb-IDQ~t7JlbdC0YJo8FbzGZQwkMbSg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fibrinogen residues γ390-396 are necessary for FXIII-A2B2 binding. Recombinant human fibrinogen variants (γA/γA, γ′/γ′, Aα251, and Fibγ390-396A) were mixed with FXIII-A2B2 (1 mg/mL [2.9 μM] and 20 µg/mL [60 nM], final, respectively) and precipitated with glycine. (A) Representative western blots for fibrinogen (Fgn), FXIII-A, and FXIII-B in the initial sample (I), pellet (P), or supernatant (S). Note that P and S samples were prepared after the addition of glycine and are therefore diluted relative to the I sample. (B) Quantitation of all blots, indicating percent of FXIII-A or FXIII-B in the pellet, relative to total FXIII in the pellet and supernatant. Bars are means ± SEM; N = 3.
