N-linked glycans within the VWF A2 domain regulate enhanced clearance. Our findings suggest that the N-linked glycan within A2 may have a specific role in modulating VWF clearance. To examine if glycans outside the A2 domain may also influence VWF survival, a fragment of VWF with the A2 domain deleted was constructed. (A) Consequently, this VWF variant (ΔA2-VWF) fails to express the N-linked glycans N1515 and N1574. (B) This VWF variant was subjected to PNGase F treatment (PNG ΔA2-VWF) to remove all remaining N-linked glycans. (C) Clearance was assessed in VWF^−/− mice as before. All results are plotted as percentage residual VWF:antigen levels relative to the amount injected. Data are presented as mean ± SEM.