Outline of the Karyogene workflow. Genomic DNA was processed to capture target loci using RNA baits and sequenced on a HiSeq 2000 sequencer as described in “Methods.” Sequencing data were mapped to the genome and analyzed through the indicated software to detect the corresponding types of mutations. The bait design underpinning these is described in supplemental Figure 1. HD, high definition; PE, paired-end.