Plasma VWF:Ag and VWF:CB levels are increased in ECM. Following intraperitoneal inoculation with 2 × 106P berghei ANKA parasites, whole blood samples were collected from WT C57BL/6J mice by cardiac puncture. (A) Plasma VWF:Ag levels were then measured at each time point by ELISA. All ELISAs were performed in triplicate, and results presented represent the mean values ± SEM unless otherwise stated (*P < .05, **P < .01, ***P < .0001, respectively). (B) Peripheral blood P berghei parasitemia levels were determined from Giemsa-stained smears (n = 16; mean values shown). (C) ECM progression was monitored using a previously validated clinical scoring algorithm (n = 16; mean values shown). (D) Plasma VWF:CB activity levels were also determined by ELISA, as detailed in the “Materials and methods” section.
