![Figure 1. EphA4 expression is decreased post-EBV infection. (A) Total RNAs were harvested from uninfected primary B cells, from peripheral blood, and EBV-immortalized LCLs from 3 different donors. cDNAs were generated using [33P]-labeled degenerate primers for PTKs. EphA4-specific cDNAs were digested by 3 restriction enzymes, BstNI, HhaI, and MnlI. Relative fold EphA4 expression was compared with primary B cells. (B-C) Peripheral CD19+ B cells were seeded at 1 × 106 cells/mL and infected with EBV strain B95.8. Total RNAs and protein were harvested from primary B cells at the days indicated post-EBV infection. (B) Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (C) EphA4, EBNA1, LMP1, and β-actin proteins were measured by western blotting. EphA4 protein-relative folds were normalized to internal control β-actin and compared with uninfected primary B cells. (D) Total RNAs were extracted from primary B cells, EBV infection, or B-cell stimulations including anti-CD40 antibody plus IL-4, LPS, or poly I:C for 3 days. EphA4 transcripts were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (E) Total RNAs were extracted from primary B cells and 8 LCL lines. Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (F-G) Total RNAs and protein were harvested from paired uninfected B cells and LCLs generated from the peripheral blood mononuclear cells of 2 healthy donors. (F) Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (G) EphA4, EBNA1, LMP1, and β-actin proteins were measured by western blotting. EphA4 protein-relative folds were normalized to β-actin and compared with uninfected primary B cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/12/10.1182_blood-2016-02-702530/4/1578f1.jpeg?Expires=1725224699&Signature=ftNOmlgXXXBdQpOqPmfL23Lx1of0nIsYCUBrB~szfdj9xZ6DP7f4oFpWbMY91lB~UHvIYNI3Z-LeZJU55HwkwKYYJBx2w~dEkNWPxRlBwvS8Of3QwD5mTIP29cZq6LHNwUqj~zeOAoGvYhfBUgagPEEpaKajnh4D2n0Nk3vpsm8IjbZ650QrAfGzYKl3dquuPOwDxc0CsBdq-11La81bNmaQTSEcRNOt~Ox-~izb-ccYJU7Sv4ttrGMzfWeTvDlkGCIMNkXD2Miv2IDOm0NOdwtbqN8oUzbkI0RyIBhWfqZobDZ2JteeUSdVU8~jOd6jVazuUDT-IzFjP8n2iAn96w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
EphA4 expression is decreased post-EBV infection. (A) Total RNAs were harvested from uninfected primary B cells, from peripheral blood, and EBV-immortalized LCLs from 3 different donors. cDNAs were generated using [33P]-labeled degenerate primers for PTKs. EphA4-specific cDNAs were digested by 3 restriction enzymes, BstNI, HhaI, and MnlI. Relative fold EphA4 expression was compared with primary B cells. (B-C) Peripheral CD19+ B cells were seeded at 1 × 106 cells/mL and infected with EBV strain B95.8. Total RNAs and protein were harvested from primary B cells at the days indicated post-EBV infection. (B) Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (C) EphA4, EBNA1, LMP1, and β-actin proteins were measured by western blotting. EphA4 protein-relative folds were normalized to internal control β-actin and compared with uninfected primary B cells. (D) Total RNAs were extracted from primary B cells, EBV infection, or B-cell stimulations including anti-CD40 antibody plus IL-4, LPS, or poly I:C for 3 days. EphA4 transcripts were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (E) Total RNAs were extracted from primary B cells and 8 LCL lines. Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (F-G) Total RNAs and protein were harvested from paired uninfected B cells and LCLs generated from the peripheral blood mononuclear cells of 2 healthy donors. (F) Expression levels of EphA4 mRNA were measured by RT-Q-PCR. EphA4 mRNA-relative folds were normalized to internal control MAGOH and standardized with uninfected primary B cells. (G) EphA4, EBNA1, LMP1, and β-actin proteins were measured by western blotting. EphA4 protein-relative folds were normalized to β-actin and compared with uninfected primary B cells.
