Requirement for autophagy in leukemia progression, stem cell maintenance, and PB leukemia cell survival in a murine CML-BC model. (A) Kaplan-Meier curves representing vehicle-treated (n = 9) and TAM-treated (n = 9) Atg7^flox/flox:Cre-ER^T2 CML-BC mice. The P value was calculated by a log-rank test. (B) Frequencies of LIC fractions (GFP⁺KusabiraOrange⁺Lineage⁻c-Kit⁺) in BM from vehicle-treated (n = 6) or TAM-treated (n = 7) Atg7^flox/flox:Cre-ER^T2 CML-BC mice were determined by flow cytometric analysis. (C) WBC counts in PB from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 CML-BC mice at day 14 after BMT were plotted (n = 6 per group). (D) Frequencies of annexin-V⁺ or DAPI⁺ cells in c-Kit⁻CD11b⁺ cells in BM or PB from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 CML-BC mice were plotted (n = 6 per group). *P < .05; **P < .01. All results represent pooled data from at least 3 independent experiments with 2 to 3 animals per experiment.