Lrp2 is required for normal definitive hematopoiesis. (A) Venn diagram shows the overlap of genes that are UP in FLK1 mesoderm enriched for BL-CFCs and/or HE cells enriched for hemogenic potential. (B) Immunohistochemistry of E10.5 Ly6aGFP AGM shows coexpression of GFP and LRP2 in ECs and hematopoietic clusters. The insets show the same sections at low magnification. (C) Homology relationships of zebrafish lrp2a and lrp2b coding sequences with that of Lrp2 in different vertebrate species. (D) ISH for the HSC marker cmyb in zebrafish at 36 hpf. (i) Low (left) and high (right) magnification images of control zebrafish (top row), lrp2 a/b morpholinos (middle row), and lrp2a/b morpholinos coinjected with hLRP2 mRNA (bottom row) zebrafish. (ii) Low (left) and high (right) magnification images of control zebrafish (top row) and lrp2b morpholinos. (E) Confocal images of flk:zsgreen reporter embryos show an intact vasculature in both control (top) and lrp2a/b morphant (bottom) embryos. DA, dorsal aorta; NC, notochord; NT, neural tube.