Retarded progression of MLL-ENL–induced murine leukemia caused by autophagy deficiency. (A) Protein levels of ATG5, ATG7, and LC3A were assessed by western blot analysis in BM cells isolated from MLL-ENL leukemic mice at day 18 post-BMT. β-actin was used as a loading control. (B-C) Kaplan-Meier curves represent vehicle- or TAM-treated Atg5^flox/flox:Cre-ER^T2 (n = 12 each) and Atg7^flox/flox:Cre-ER^T2 (n = 15 each) MLL-ENL leukemic mice. The P value was calculated by a log-rank test. (D) BM cellularities of vehicle- and TAM-treated Atg7^flox/flox:Cre-ER^T2 leukemic mice are shown (n = 13). The box depicted in the upper and lower quartiles, and the median, are signified by thick lines within the box. The whiskers represent maximum or minimum data within the 1.5× interquartile range from the top or the bottom of the box, respectively. Each circle represents an individual animal. *P < .05; ***P < .001. All results except for western blotting are pooled data from at least 3 independent experiments with 1 to 4 animals per experiment.