American Society of Hematology

Figure 2

$Macrophages are the principal cells mediating extravascular fibrin endocytosis in vivo. (A-B) Endocytic fibrin uptake by F4/80-positive cells. (A) Percentage F4/80-positive (green bar) and F4/80-negative (gray bar) cells in fibrin implantation zones. Crosshatched bars embedded within green and gray bars show fraction of, respectively, F4/80-positive and F4/80-negative cells that endocytose fibrin. Whole-mount immunostaining was performed 24 hours after fibrin implantation. Data were generated from serial analysis of confocal z-stacks from 3 mice as described in “Materials and methods.” Error bars indicate standard deviation. (B) Example of a F4/80-positive (red, white in top right panel) fibrin-internalizing (green, white in bottom left panel) cell. (C-D) Endocytic fibrin-uptake by NIMP.R14-positive cells. (C) Percentage of NIMP.R14-positive (green bar) and NIMP.R14-negative (gray bar) cells in fibrin implantation zones. Crosshatched bars embedded in green and gray bars show fraction of, respectively, NIMP.R14-positive and NIMP.R14-negative cells that endocytose fibrin. Whole-mount immunostaining was performed 24 hours after subcutaneous fibrin implantation. Data were generated as in panel A from 3 mice. (D) Example of a NIMP.R14-positive (green, white in top right panel), fibrin-negative cell, and examples of NIMP.R14-negative fibrin-positive (red, white in top panel) cells. (E-F) Endocytic fibrin-uptake by Col1a1-GFP–positive cells. Percentage of GFP-positive (green bar) and GFP-negative (gray bar) cells in implantation zones from fibrin-implanted Col1a1-GFP+/0 transgenic mice. Crosshatched bars embedded in green and gray bars show fraction of, respectively, GFP-positive and GFP-negative cells that endocytose fibrin. Data were generated as in panel A from 3 mice. (F) Example of an GFP-positive (green, white in top right panel) fibrin-internalizing (red, white in bottom left panel) cell. (G-H) Pie charts showing the constitution of all cells (G) and fibrin-internalizing cells (H) in the fibrin implantation zone.$

Macrophages are the principal cells mediating extravascular fibrin endocytosis in vivo. (A-B) Endocytic fibrin uptake by F4/80-positive cells. (A) Percentage F4/80-positive (green bar) and F4/80-negative (gray bar) cells in fibrin implantation zones. Crosshatched bars embedded within green and gray bars show fraction of, respectively, F4/80-positive and F4/80-negative cells that endocytose fibrin. Whole-mount immunostaining was performed 24 hours after fibrin implantation. Data were generated from serial analysis of confocal z-stacks from 3 mice as described in “Materials and methods.” Error bars indicate standard deviation. (B) Example of a F4/80-positive (red, white in top right panel) fibrin-internalizing (green, white in bottom left panel) cell. (C-D) Endocytic fibrin-uptake by NIMP.R14-positive cells. (C) Percentage of NIMP.R14-positive (green bar) and NIMP.R14-negative (gray bar) cells in fibrin implantation zones. Crosshatched bars embedded in green and gray bars show fraction of, respectively, NIMP.R14-positive and NIMP.R14-negative cells that endocytose fibrin. Whole-mount immunostaining was performed 24 hours after subcutaneous fibrin implantation. Data were generated as in panel A from 3 mice. (D) Example of a NIMP.R14-positive (green, white in top right panel), fibrin-negative cell, and examples of NIMP.R14-negative fibrin-positive (red, white in top panel) cells. (E-F) Endocytic fibrin-uptake by Col1a1-GFP–positive cells. Percentage of GFP-positive (green bar) and GFP-negative (gray bar) cells in implantation zones from fibrin-implanted Col1a1-GFP^+/0 transgenic mice. Crosshatched bars embedded in green and gray bars show fraction of, respectively, GFP-positive and GFP-negative cells that endocytose fibrin. Data were generated as in panel A from 3 mice. (F) Example of an GFP-positive (green, white in top right panel) fibrin-internalizing (red, white in bottom left panel) cell. (G-H) Pie charts showing the constitution of all cells (G) and fibrin-internalizing cells (H) in the fibrin implantation zone.

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