CM expression alters the proliferation and apoptosis of preleukemic Pre-Meg/E progenitor population. (A) Representative FACS plots of Ki67/DAPI (left) or Annexin-V/DAPI (right) staining and gating strategy in CM or control BM HSPCs. (B) Frequency (%) of CM preleukemic (n = 6) or control (n = 6) LSK, Pre-GM, GMP, Pre-Meg/E, and EP in G0, G1, or S/G2/M phases of cell cycle as defined above. Bar graphs and lines indicate mean + standard deviation (SD). (C) The frequency (%) of apoptotic cells defined by Annexin-V⁺ in CM (n = 6) or Ctrl (n = 5) LSK, Pre-GM, GMP, Pre-Meg/E, and EP. Bars and lines indicate mean + SD; *P < .05; **P < .01; ***P < .001.