Anti-IgM regulation of MYC mRNA transcription and translation. CLL samples were pretreated for 1 hour with ibrutinib or tamatinib, or DMSO as a control, and then incubated with anti-IgM or control beads for 24 hours. (A-C) Monosome- and polysome-associated MYC mRNA was quantified using quantitative polymerase chain reaction; the graphs show total MYC mRNA (monosomal plus polysomal) (A), polysome-associated MYC mRNA (B), and polysome/monosome ratio for MYC mRNA (C) for ibrutinb (n = 5) and tamatinib (n = 6). (D-E) MYC and β-actin (loading control) protein analysis by immunoblotting. (D) Representative immunoblots and (E) quantitation of multiple experiments for 4 samples. The graphs show mean fold increases (± SEM), with values for anti–IgM/DMSO-treated cells set at 100%. Statistical comparisons between anti–IgM/DMSO-treated cells are shown (Student t test).