IL-15 primes a stress-induced gene expression program in NK cells. (A) A heatmap of mean polysome-association (log2) per condition (A = activated, W = cytokine withdrawal) for mRNAs showing differential (IL-15 vs IL-2) polysome association after cytokine withdrawal. Clustering was performed using data from this set of mRNAs across all 4 conditions. The right sidebar indicates the identified gene subsets; the left sidebar shows proportions of genes regulated by differential translation (anota) per subset. Clusters corresponding to induced or primed modes of regulation are indicated. Across genes mean ± SD per condition and cluster is also plotted. (B) A heatmap illustrating results from enrichment analysis within subsets identified in panel A for cellular processes defined by GO. The color scale indicates significance for enrichment for GO terms identified as enriched in at least 1 subset (FDR <0.1 and odds ratio >1.5). (C) Freshly isolated human primary NK cells were activated (A) with IL-2 or IL-15 (18.3 ng/mL) for 48 hours with or without torin-1 (1 µM) and subsequently deprived of cytokines for 24 hours (W). Phosphorylation of the mTOR target S6 kinase was determined by western blot and compared with total S6K. Actin served as a loading control.