Differentiation characteristics of CD4Tregs, CD4Tcons, and CD8 T cells associated with cGVHD. Percentages of naive, CM, and EM subsets were determined at 3 and 6 months after transplant. Results were compared in patients who developed (red) or did not develop (blue) cGVHD. (A) CM:EM ratio in CD4Tregs. (B) CM:EM ratio in CD4Tcons. (C) CD4Tregs: Median percentage of the naive, CM, and EM. (D) CD4Tcons: Median percentage of the naive, CM, and EM. (E) Frequency profile of the entire T-cell compartment at 3 months after transplant. Data are presented as a heatmap using hierarchical clustering after standardization of each row. Top bar indicates subsequent cGVHD outcome (green, cGVHD; yellow, no cGVHD). Blue, white, and red represent low, intermediate, and high subset frequency.