Tissue distribution of CD3⁺ T cells after rhIL-7 treatment. Eight study patients and 17 HCs underwent flexible sigmoidoscopy with rectosigmoid biopsy for immunohistochemical analysis. (A) Representative image of CD3⁺ staining of the lamina propria in a study patient before and after rIL-7 treatment. The black arrows indicate areas of positive CD3 staining. (B) Percentage of lamina propria surface area staining positive for CD3 in each of the 8 patients with rectosigmoid biopsies before (week 0) and after (week 24) 3 doses of rhIL-7 treatment as well as the values for the HCs. Each patient’s results are depicted by color and symbol, as in Figure 1. HCs are depicted as black diamonds, and the straight horizontal line shows the median value for the HCs. (C) Seven study patients and 10 HCs underwent bone marrow biopsy for immunohistochemical staining for CD3. (C) Representative image of CD3⁺staining in the bone marrow before and after rIL-7 in a study patient. The black arrows indicate areas of positive CD3 staining. (D) Mean number of CD3⁺ cells per high power field for each of the 7 patients with bone marrow biopsies before and after rhIL-7 treatment. The color scheme and symbols are the same as those used in (B).