Polyfunctionality of CD4 and CD8 T cells after rhIL-7 treatment. CD4 and CD8 T cells from HCs (n = 6) and study patients (n = 5) at weeks 0, 12, and 48 were stimulated with phorbol myristate acetate (PMA) and ionomycin. Stimulated cells were then analyzed by flow cytometry for the production of intracellular cytokines IL-2, tumor necrosis factor α (TNF-α), IL-17α, and interferon gamma (IFN-γ). Pie charts depict median values for HCs or individual values for each study patient at each time point, demonstrating increasing polyfunctionality (ie, increasing numbers of cytokines. The bar graphs adjacent to each pie chart demonstrate the proportion of CD4 T cells producing IL-2, TNF-α, IL-17α, and IFN-γ at the corresponding time point.