miRNA profiling of expanded naïve CD4+ T cells and naive tTregs demonstrates stronger expression of miR-146b in human tTregs (n = 3). Naive T cells (CD4⁺25-127⁺45RA⁺) and naive tTregs (CD4⁺25⁺⁺127-45RA⁺) were sort-purified and expanded in vitro. miRNA expression in control T cells (ctrl T) and tTregs was determined by miRNA TaqMan Low Density Array. (A) After analyzing 768 miRNAs, the top 10 differential miRNAs between tTregs and control T cells were gated for further analysis by heatmap (left) and average (AVG) fold differential expression (right) (P < .05) . (B) Relative (Rel.) differential expression of miR-146b in control T cells and tTregs (n = 3) was confirmed by RT-PCR *P < .05.