tfec^−/−mutants have reduced hematopoiesis and become anemic. (A-B) ISH for cmyb and gata1 at 4 dpf in WT/tfec^+/− embryos or tfec^−/− mutant embryos. Sib. represents WT/tfec^+/− genotype. (C) Brightfield image at 9 dpf showing tfec^−/− mutants and their siblings. Arrows indicate normal pigmented eye in siblings and inflated swim bladder; asterisks indicate loss of eye pigmentation and uninflated swim bladder in mutants. (D) Circulating blood phenotype at 8 and 9 dpf characterized by normal blood flow (normal), low circulating number of cells (low), and almost complete loss of circulating cells (anemia).