Landscape of mutations. (A) Distribution of variant allele frequencies of SF3B1, TET2, ASXL1, and DNMT3A gene mutations at inclusion. For each mutation, depth at the variant position was considered to calculate variant allele frequency; that is, the proportion of mutated reads among total reads and its 95% CI. For each patient having at least 1 of the 4 SF3B1, TET2, ASXL1, or DNMT3A mutations, pairwise comparisons between the variant allele frequencies of mutations were performed using Fisher’s exact test. Mutations with the significantly highest variant allele frequencies were considered major mutations and are indicated by closed symbols; minor mutations are indicated by open symbols. (B) Barcode representation of genetic lesions, cytogenetic abnormalities, and response status of 94 patients. Two patients had no mutation and a normal karyotype. Each column represents an individual sample, and each row represents a gene. Black boxes represent abnormal karyotypes and responders to treatment. Gray boxes represent unavailable karyotype.