NE and EPI facilitate PPF. (A) Representative photographs of PPF after NE (1 μM) or EPI (1 μM) stimulation. Cytoskeleton actin (green) and the nucleus (blue) were stained. (B-C) Quantification of proplatelet-forming MKs treated with NE or EPI at different concentrations under an inverted microscope. (D-E) Western blot analysis of the expression of the phospho-RhoA in MKs prestimulated with yohimbine, prazosin, or U0126 for 20 minutes followed by NE or EPI treatment of 30 minutes. (F) Morphology exhibition of MKs treated with 10 μM of the ROCK inhibitor Y27632 for 20 minutes before stimulation with NE or EPI. Cytoskeleton actin (green), tubulin (red), and the nucleus (blue) were stained. (G-H) The counts of proplatelet-forming MKs exposed to 10 μM prazosin, 10 μM yohimbine, or 10 μM U0126 prior to NE or EPI stimulation. *P < .05, **P < .01, vs control; #P < .05, ##P < .01, vs NE or EPI treatment group.