NE and EPI promote the adherence and migration of MKs. The progeny of CD34⁺ cells cultured with rhTPO for 13 days were purified first and then used for the experiments. (A-B) Adherent and migrated MKs treated with different concentrations of NE or EPI. (C-D) Adherence and migration of MKs pretreated with phentolamine (10 μM) or propranolol (10 μM) for 20 minutes before incubation with 1 μM NE or EPI. (E) Western blot analysis for ERK1/2 phosphorylation in MKs after exposure to NE (1 μM) or EPI (1 μM) for the indicated times. (F) ERK1/2 phosphorylation in MKs pretreated with different adrenergic antagonists for 20 minutes followed by NE (1 μM) or EPI (1 μM) treatment of 30 minutes. (G-H) The inhibitory effect of yohimbine, prazosin, and U0126 on the adherence and migration of MKs induced by NE (1 μM) or EPI (1 μM). *P < .05, **P < .01, vs control; #P < .05, ##P < .01, vs NE or EPI treatment group.