Spectrum and correlation of RHOA mutations in ATLL. (A) Mutational status of RHOA, TET2, IDH2, and DNMT3A genes in 203 ATLL cases. Subtype of each case is shown by indicated colors. (B) Comparison of distributions of RHOA and TET2 mutations between AITL and ATLL. Co-existence of RHOA and TET2 mutations are shown in AITL and ATLL. Red and blue circles indicated cases with RHOA and TET2 mutations, respectively. (C) Comparison of VAFs between RHOA and TET2 mutations. ATLL cases with both TET2 and RHOA mutations (n = 5) were analyzed. Each axis shows the VAFs of mutations. VAFs with genomic copy number abnormalities were corrected according to the usual methods. P value was calculated by Mann–Whitney U test. (D) Frequency of CNVs at the RHOA locus in ATLL cases with or without RHOA mutations. P value was calculated by Fisher’s exact test. (E) VAFs of RHOA mutations in cases with or without CNVs at the RHOA locus. Only patients with a single RHOA mutation were considered. P value was determined using Student t test.