cAMP-mediated changes in GIE deformability are linked to STEVOR expression. (A-B) Retention in microsphilters of stages III (A) and stages V (B) GIE from the B10 (dark gray) and the A12 (medium gray) wild-type clones preincubated at 37°C during 30 minutes with (+cAMP) or without (–cAMP) 100 µM 8Br-cAMP. (C-D) Retention in microsphilters of stages III (C) and stages V (D) GIE from the control (dark gray) and the Null (medium gray) parasite line. GIEs were preincubated at 37°C 30 minutes with (+cAMP) or without (−cAMP) 100 µM 8Br-cAMP. Error bars denote the standard error of the mean. Outliers are shown as open circles. Stars represent significant differences in retention rates (***P < .001; **P < .01; *P < .05). ns, nonsignificant differences in retention rates.